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Computational thermal analysis of a continuous flow micro Polymerase Chain Reaction (PCR) chip
conference contribution
posted on 2006-01-01, 00:00 authored by D Sugumar, M Ashraf, Lingxue KongLingxue KongThe first continuous flow micro PCR introduced in 1998 has attracted considerable attention for the past several years because of its ability to amplify DNA at much faster rate than the conventional PCR and micro chamber PCR method. The amplification is obtained by moving the sample through 3 different fixed temperature zones. In this paper, the thermal behavior of a continuous flow PCR chip is studied using commercially available finite element software. We study the temperature uniformity and temperature gradient on the chip’s top surface, the cover plate and the interface of the two layers. The material for the chip body and cover plate is glass. The duration for the PCR chip to achieve equilibrium temperature is also studied.
History
Event
International Society for Optical Engineering (2006 : Adelaide, S.Aust.)Publisher
SPIE, International Society for Optical EngineeringLocation
Adelaide, S. Aust.Place of publication
Bellingham, Wash.Start date
2006-12-01End date
2006-12-13ISBN-13
9780819465238ISBN-10
0819465232Language
engPublication classification
E1.1 Full written paper - refereedEditor/Contributor(s)
J Chiao, A Dzurak, C Jagadish, D ThielTitle of proceedings
SPIE 2006 : Proceedings : Micro and Nanotechnology : Materials, Processes, Packaging, and Systems IIIUsage metrics
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