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AFLP fingerprinting of the human genome

journal contribution
posted on 2001-01-01, 00:00 authored by M Prochazka, Ken WalderKen Walder, J Xia
Elucidation of the genetic basis of complex traits and diseases in humans includes the use of genome-wide association studies that depend on the analysis of a large number of diallelic markers. We describe the application of the amplified fragment length polymorphism (AFLP) technique as an efficient approach for rapidly identifying and scoring multiple variants in the human genome. Using a commercially available kit, we found that AFLP yields reproducible DNA fingerprints consisting of 42-132 fragments, 8% of which show variability between individuals. These variant markers appear to be from different chromosomes, and the majority of them is diallelic. Based on the information obtained in this study, it is possible to approximate the minimum number of selective AFLP primer combinations needed to approach a desired coverage density of all chromosomes. To our knowledge, this is the first study showing the general applicability of AFLP in humans and providing a constructive guide for the design of genomic studies in Homo sapiens with this robust methodology.

History

Journal

Human genetics

Volume

108

Issue

1

Pagination

59 - 65

Publisher

Springer Verlag

Location

Berlin, Germany

ISSN

0340-6717

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2000, Springer-Verlag

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