francis-n3pufadepletion-2017.pdf (597.19 kB)
A n-3 PUFA depletion applied to rainbow trout fry (Oncorhynchus mykiss) does not modulate its subsequent lipid bioconversion capacity
journal contribution
posted on 2017-01-01, 00:00 authored by J Mellery, J Brel, J Dort, F Geay, P Kestemont, David FrancisDavid Francis, Y Larondelle, X RollinNutritional strategies are currently developed to produce farmed fish rich in n-3 long-chain PUFA (LC-PUFA) whilst replacing fish oil by plant-derived oils in aquafeeds. The optimisation of such strategies requires a thorough understanding of fish lipid metabolism and its nutritional modulation. The present study evaluated the fatty acid bioconversion capacity of rainbow trout (Oncorhynchus mykiss) fry previously depleted in n-3 PUFA through a 60-d pre-experimental feeding period with a sunflower oil-based diet (SO) followed by a 36-d experimental period during which fish were fed either a linseed oil-based diet (LO) (this treatment being called SO/LO) or a fish oil-based diet (FO) (this treatment being called SO/FO). These treatments were compared with fish continuously fed on SO, LO or FO for 96 d. At the end of the 36-d experimental period, SO/LO and SO/FO fish recovered >80 % of the n-3 LC-PUFA reported for LO and FO fish, respectively. Fish fed on LO showed high apparent in vivo elongation and desaturation activities along the n-3 biosynthesis pathway. However, at the end of the experimental period, no impact of the fish n-3 PUFA depletion was observed on apparent in vivo elongation and desaturation activities of SO/LO fish as compared with LO fish. In contrast, the fish n-3 PUFA depletion negatively modulated the n-6 PUFA bioconversion capacity of fish in terms of reduced apparent in vivo elongation and desaturation activities. The effects were similar after 10 or 36 d of the experimental period, indicating the absence of short-term effects.
History
Journal
British journal of nutritionVolume
117Issue
2Pagination
187 - 199Publisher
Cambridge University PressLocation
Cambridge, Eng.Publisher DOI
eISSN
1475-2662Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2017, The AuthorsUsage metrics
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No categories selectedKeywords
ALA α-linolenic acidCD coefficient of distanceDGC daily growth coefficientFE feed efficiencyFO fish oil-based dietLA linoleic acidLC-PUFA long-chain PUFALO linseed oil-based dietSO sunflower oil-based dietSO\/FO SO until day 60 and then FO from days 61–96SO\/LO SO until day 60 and then LO from days 61–96Fatty acid metabolismLipid bioconversion capacityPlant-derived oilsRainbow troutWhole body fatty acid balance methodAnimal FeedAnimalsAquacultureDietFatty Acid DesaturasesFatty Acids, Omega-3Fatty Acids, Omega-6Fish OilsLinseed OilLipid MetabolismMalnutritionOncorhynchus mykissPlant OilsScience & TechnologyLife Sciences & BiomedicineNutrition & DieteticsFATTY-ACID-COMPOSITIONSALMO-SALAR L.DIETARY FISH-OILGROW-OUTIN-VIVOOXIDATIVE STABILITYDESATURASE ACTIVITYVEGETABLE-OILSPROFILE CHANGEALA \n α-linolenic acid
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