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A surface-stress-based microcantilever aptasensor
journal contribution
posted on 2014-01-01, 00:00 authored by Yang Lim, Abbas KouzaniAbbas Kouzani, Wei DuanWei Duan, Xiujuan Dai, Akif KaynakAkif Kaynak, D MairBiosensors based on microcantilevers convert biological recognition events into measurable mechanical displacements. They offer advantages such as small size, low sample volume, label-free detection, ease of integration, high-throughput analysis, and low development cost. The design and development of a microcantilever-based aptasensor employing SU-8 polymer as the fabrication material is presented in this paper. Aptamers are employed as bioreceptor elements because they exhibit superior specificity compared to antibodies due to their small size and physicochemical stability. To immobilise thrombin DNA aptamer on the bare SU-8 surface of the aptasensor, a combined plasma mode treatment method is implemented which modifies the surface of the aptasensor. Label-free detection of thrombin molecules using the fabricated aptasensor is successfully demonstrated. The measured deflection is one order of magnitude higher than that of a silicon nitride microcantilever biosensor. The developed aptasensor also demonstrates high specificity.
History
Journal
IEEE transactions on biomedical circuits and systemsVolume
8Issue
1Pagination
15 - 24Publisher
IEEELocation
Piscataway, NJPublisher DOI
ISSN
1932-4545Language
engPublication classification
C1 Refereed article in a scholarly journal; C Journal articleCopyright notice
2014, IEEEUsage metrics
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