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Acquisition of invasion‐inhibitory antibodies specific for the 19‐kDa fragment of merozoite surface protein 1 in a transmigrant population requires multiple infections
journal contribution
posted on 2008-10-01, 00:00 authored by E Murhandarwati, C Black, L Wang, S Weisman, Tania De Koning-WardTania De Koning-Ward, J Baird, E Tjitra, T Richie, B Crabb, R CoppelAntibodies against the 19 kDa C‐terminal fragment of merozoite surface protein 1 (MSP119) are a major component of the invasion‐inhibitory response in individuals immune to malaria. We report here the acquisition of MSP119‐specific invasion‐inhibitory antibodies in a group of transmigrants who experienced their sequential malaria infections during settlement in an area of Indonesia where malaria is highly endemic. We used 2 transgenic Plasmodium falciparum parasite lines that expressed either endogenous MSP119 or the homologous region from P. chabaudi to measure the MSP119‐specific invasion‐inhibitory antibodies. The results revealed that the acquisition of MSP119‐specific invasion‐inhibitory antibodies required 2 or more P. falciparum infections. In contrast, enzyme‐linked immunosorbent assays on the same serum samples showed that MSP119‐specific antibodies are present after the first malaria infection. This delay in the acquisition of functional antibodies by residents of areas where malaria is endemic is consistent with the observation that multiple malaria infections are required before clinical immunity is acquired.
History
Journal
The journal of infectious diseasesVolume
198Issue
8Pagination
1212 - 1218Publisher
University of Chicago PressLocation
Chicago, Ill.Publisher DOI
ISSN
0022-1899eISSN
1537-6613Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2008, Infectious Diseases Society of AmericaUsage metrics
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