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Activation of the selenopritein SEPS1 gene expression by pro-inflammatory cytokines in Hep G2 cells

journal contribution
posted on 2006-03-07, 00:00 authored by Yuan Gao, N Hannan, Stephen Wanyonyi, Nicky Konstantopoulos, J Pagnon, Helen Feng, Jeremy Jowett, K H Kim, Ken WalderKen Walder, Gregory Collier
SEPS1 (also called selenoprotein S, SelS) plays an important role in the production of inflammatory cytokines and its expression is activated by endoplasmic reticulum (ER) stress. In this report, we have identified two binding sites for the nuclear factor kappa B in the human SEPS1 promoter. SEPS1 gene expression, protein levels and promoter activity were all increased 2–3-fold by TNF-α and IL-1β in HepG2 cells. We have also confirmed that the previously proposed ER stress response element GGATTTCTCCCCCGCCACG in the SEPS1 proximate promoter is fully functional and responsive to ER stress. However, concurrent treatment of HepG2 cells with IL-1β and ER stress produced no additive effect on SEPS1 gene expression. We conclude that SEPS1 is a new target gene of NF-κB. Together with our previous findings that SEPS1 may regulate cytokine production in macrophage cells, we propose a regulatory loop between cytokines and SEPS1 that plays a key role in control of the inflammatory response.

History

Journal

Cytokine

Volume

33

Issue

5

Pagination

246 - 251

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

1043-4666

eISSN

1096-0023

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2006, Elsevier Ltd