gupta-amyloidinduces-2019.pdf (5.49 MB)
Amyloid β induces early changes in the ribosomal machinery, cytoskeletal organization and oxidative phosphorylation in retinal photoreceptor cells
journal contribution
posted on 2019-02-22, 00:00 authored by L Deng, K Pushpitha, C Joseph, Veer GuptaVeer Gupta, R Rajput, N Chitranshi, Y Dheer, A Amirkhani, K Kamath, D Pascovici, J X Wu, G H Salekdeh, P A Haynes, S L Graham, V K Gupta, M MirzaeiAmyloid β (Aβ) accumulation and its aggregation is characteristic molecular feature of the development of Alzheimer’s disease (AD). More recently, Aβ has been suggested to be associated with retinal pathology associated with AD, glaucoma and drusen deposits in age related macular degeneration (AMD). In this study, we investigated the proteins and biochemical networks that are affected by Aβ in the 661 W photoreceptor cells in culture. Time and dose dependent effects of Aβ on the photoreceptor cells were determined utilizing tandem mass tag (TMT) labeling-based quantitative mass-spectrometric approach. Bioinformatic analysis of the data revealed concentration and time dependent effects of the Aβ peptide stimulation on various key biochemical pathways that might be involved in mediating the toxicity effects of the peptide. We identified increased Tau phosphorylation, GSK3β dysregulation and reduced cell viability in cells treated with Aβ in a dose and time dependent manner. This study has delineated for the first-time molecular networks in photoreceptor cells that are impacted early upon Aβ treatment and contrasted the findings with a longer-term treatment effect. Proteins associated with ribosomal machinery homeostasis, mitochondrial function and cytoskeletal organization were affected in the initial stages of Aβ exposure, which may provide key insights into AD effects on the photoreceptors and specific molecular changes induced by Aβ peptide.
History
Journal
Frontiers in Molecular NeuroscienceVolume
12Article number
24Pagination
1 - 14Publisher
Frontiers MediaLocation
Lausanne, SwitzerlandPublisher DOI
Link to full text
ISSN
1662-5099eISSN
1662-5099Language
EnglishPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2019, Deng, Pushpitha, Joseph, Gupta, Rajput, Chitranshi, Dheer, Amirkhani, Kamath, Pascovici, Wu, Salekdeh, Haynes, Graham, Gupta and MirzaeiUsage metrics
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No categories selectedKeywords
Science & TechnologyLife Sciences & BiomedicineNeurosciencesNeurosciences & NeurologyproteomicsTMTAlzheimer's diseasephotoreceptorautophagyamyloidretinaALZHEIMERS-DISEASE IMPLICATIONSMITOCHONDRIAL DYSFUNCTIONMACULAR DEGENERATIONPRECURSOR PROTEINTAUPEPTIDEDAMAGEPATHOLOGIESNEUROTOXICITYACCUMULATIONAlzheimer’s disease
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