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Apparent in vivo Δ-6 desaturase activity, efficiency, and affinity are affected by total dietary C18 PUFA in the freshwater fish Murray cod
journal contribution
posted on 2009-05-27, 00:00 authored by David FrancisDavid Francis, D Peters, Giovanni TurchiniGiovanni TurchiniDietary fatty acids are known to modulate fatty acid metabolism in fish. However, the innate capability of fish to bioconvert short chain fatty acids to health promoting long chain fatty acids (LCPUFA) is insufficient to compensate for a reduced dietary intake. While many studies have focused on the dietary regulation of the fatty acid bioconversion pathways, there is little known regarding the effects of the dietary levels of C18 polyunsaturated fatty acids (PUFA) on fatty acid metabolism. Here, we show a greater degree of apparent enzyme activity (Δ-6 desaturase) in fish fed a diet with higher amounts of dietary C18 PUFA. In particular, fish receiving high amounts of dietary C18 PUFA had a greater amount of Δ-6 desaturase activity acting on 18:3n-3 than 18:2n-6. However, with the gradual reduction of dietary C18 PUFA there was a shift in substrate preference of Δ-6 desaturase from 18:3n-3 to 18:2n-6. This information will provide valuable insight for the implementation of low fish oil diets, which permit the maintenance of n-3 LCPUFA levels in farmed Murray cod.
History
Journal
Journal of agricultural and food chemistryVolume
57Issue
10Pagination
4381 - 4390Publisher
American Chemical SocietyLocation
United StatesPublisher DOI
ISSN
0021-8561eISSN
1520-5118Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2009, American Chemical SocietyUsage metrics
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Maccullochella peeli peelienzyme affinityΔ-6 desaturasefatty acidlinoleic acidα-linolenic acidScience & TechnologyLife Sciences & BiomedicinePhysical SciencesAgriculture, MultidisciplinaryChemistry, AppliedFood Science & TechnologyAgricultureChemistryMaccullochella peelii peeliiDelta-6 desaturasealpha-linolenic acidMACCULLOCHELLA-PEELII-PEELIIFATTY-ACID-COMPOSITIONTROUT ONCORHYNCHUS-MYKISSDIFFERENT LIPID SOURCESOIL FINISHING DIETRAINBOW-TROUTGROWTH-PERFORMANCEINCREASING LEVELSBETA-OXIDATIONVEGETABLE-OILS
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