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Application of the Ceditest® FMDV type O and FMDV-NS enzyme-linked immunosorbent assays for detection of antibodies against foot-and-mouth disease virus in selected livestock and wildlife species in Uganda
journal contribution
posted on 2012-03-01, 00:00 authored by C Ayebazibwe, F N Mwiine, S N Balinda, K Tjørnehøj, Soren AlexandersenSoren AlexandersenDiagnosis and control of Foot-and-mouth disease virus (FMDV) requires rapid and sensitive diagnostic tests. Two antibody enzyme-linked immunosorbent assay (ELISA) kits, Ceditest® FMDV-NS for the detection of antibodies against the nonstructural proteins of all FMDV serotypes and Ceditest® FMDV type O for the detection of antibodies against serotype O, were evaluated under African endemic conditions where the presence of multiple serotypes and the use of nonpurified vaccines complicate serological diagnosis. Serum samples from 218 African buffalo, 758 cattle, 304 goats, and 88 sheep were tested using both kits, and selected samples were tested not only in serotype-specific ELISAs for antibodies against primarily FMDV serotype O, but also against other serotypes. The FMDV-NS assay detected far more positive samples (93%) than the FMDV type O assay (30%) in buffalo (P < 0.05), with predominant antibodies against the South African Territories (SAT) serotypes, while the seroprevalence was generally comparable in cattle with antibodies against serotype O elicited by infection and/or vaccination. However, some districts had higher seroprevalence using the FMDV type O assay indicating vaccination without infection, while 1 cattle herd with antibodies against the SAT serotypes had far more positive samples (85%) using the FMDV-NS versus the FMDV type O (10%), consistent with the latter test's lower sensitivity for antibodies against SAT serotypes. Based on the current investigation, the FMDV type O ELISA may be limited by the presence of SAT serotypes. The FMD NS assay worked well as a screening test for antibodies against all FMDV serotypes present in Uganda; however, as long as nonpurified vaccines are applied in the region, this test cannot be used to differentiate between vaccinated and infected animals.
History
Journal
Journal of veterinary diagnostic investigationVolume
24Issue
2Pagination
270 - 276Publisher
Sage PublicationsLocation
London, Eng.Publisher DOI
Link to full text
ISSN
1943-4936eISSN
1943-4936Language
engPublication classification
C Journal article; C1.1 Refereed article in a scholarly journalCopyright notice
2012, SageUsage metrics
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Keywords
AnimalsAnimals, WildAntibodies, ViralChi-Square DistributionEnzyme-Linked Immunosorbent AssayFoot-and-Mouth DiseaseFoot-and-Mouth Disease VirusLivestockReagent Kits, DiagnosticUgandaScience & TechnologyLife Sciences & BiomedicineVeterinary SciencesCeditest (R) FMDV-NSCeditest (R) FMDV type OBUFFALOS SYNCERUS-CAFFERNONSTRUCTURAL PROTEINSEXPERIMENTAL INFECTIONSMALL RUMINANTSCATTLEVACCINATIONDIFFERENTIATIONANTIGENSAFRICAELISAZoology
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