alshamaileh-aptamermediated-2017.pdf (2.83 MB)
Aptamer-mediated survivin RNAi enables 5-fluorouracil to eliminate colorectal cancer stem cells
journal contribution
posted on 2017-07-19, 00:00 authored by H Al Shamaileh, T Wang, D Xiang, Wang Yin, Phuong TranPhuong Tran, R A Barrero, P-Z Zhang, Y Li, Lingxue KongLingxue Kong, K Liu, S-F Zhou, Y Hou, Sarah ShigdarSarah Shigdar, Wei DuanWei DuanThe development of chemoresistance and inability in elimination of cancer stem cells are among the key limitations of cancer chemotherapy. Novel molecular therapeutic strategies able to overcome such limitations are urgently needed for future effective management of cancer. In this report, we show that EpCAM-aptamer-guided survivin RNAi effectively downregulated survivin both in colorectal cancer cells in vitro and in a mouse xenograft model for colorectal cancer. When combined with the conventional chemotherapeutic agents, the aptamer-guided survivin RNAi was able to enhance the sensitivity towards 5-FU or oxaliplatin in colorectal cancer stem cells, increase apoptosis, inhibit tumour growth and improve the overall survival of mice bearing xenograft colorectal cancer. Our results indicate that survivin is one of the key players responsible for the innate chemoresistance of colorectal cancer stem cells. Thus, aptamer-mediated targeting of survivin in cancer stem cells in combination with chemotherapeutic drugs constitutes a new avenue to improve treatment outcome in oncologic clinics.
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Journal
Scientific reportsVolume
7Issue
1Article number
5898Pagination
1 - 9Publisher
Nature Publishing GroupLocation
London, Eng.Publisher DOI
eISSN
2045-2322Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2017, The AuthorsUsage metrics
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