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Assessing health risk of reclaimed water using human cell culture.
journal contribution
posted on 2010-12-01, 00:00 authored by Leigh AcklandLeigh Ackland, Agnes MichalczykAgnes Michalczyk, David Freestone, Francesco StagnittiFor risk analysis of reclaimed water, current animal and toxicity testing may not detect subtle effects such as interactions that could contribute to complex diseases such as cancers that develop over a long period of time. There is a need for assays that can be validated against known human physiological processes. We have previously validated sensitive human cell culture assays for their responsiveness to agents that induce carcinogenesis in vivo. In this initial study we analysed the effects of three batches of reclaimed water on human colonic cells. At concentrations of up to 10-fold, they had no significant effect on the cellular markers, indicating an overall lack of biological activity. The assay has potential but needs to be refined to maximise its sensitivity.
History
Journal
WaterVolume
37Issue
8Pagination
39 - 43Publisher
Australian Water AssociationLocation
St Leonards, N.S.W.ISSN
0310-0367Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2010, Australian Water AssociationUsage metrics
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