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Beacon interacts with cdc2/cdc28-like kinases
journal contribution
posted on 2003-04-25, 00:00 authored by Lakshmi Kantham, L Kerr-Bayles, Nathan Godde, Melissa Quick, R Webb, T Sunderland, J Bond, Ken WalderKen Walder, G Augert, Gregory CollierPreviously we found elevated beacon gene expression in the hypothalamus of obese Psammomys obesus. Beacon administration into the lateral ventricle of P. obesus stimulated food intake and body weight gain. In the current study we used yeast two-hybrid technology to screen for proteins in the human brain that interact with beacon. CLK4, an isoform of cdc2/cdc28-like kinase family of proteins, was identified as a strong interacting partner for beacon. Using active recombinant proteins and a surface plasmon resonance based detection technique, we demonstrated that the three members of this subfamily of kinases (CLK1, 2, and 4) all interact with beacon. Based on the known sequence and functional properties of beacon and CLKs, we speculate that beacon could either modulate the function of key regulatory molecules such as PTP1B or control the expression patterns of specific genes involved in the central regulation of energy metabolism.
History
Journal
Biochemical and biophysical research communicationsVolume
304Issue
1Pagination
125 - 129Publisher
Elsevier ScienceLocation
New York, N.Y.Publisher DOI
ISSN
0006-291XeISSN
1090-2104Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2003, Elsevier Science (USA)Usage metrics
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No categories selectedKeywords
beaconCLKcdc2\/cdc28-like kinaseobesityprotein\/protein interactionsSPRyeast two-hybridubiquitinsentrinScience & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiophysicsTYROSINE-PHOSPHATASE 1BSIGNAL-TRANSDUCTIONINSULIN SENSITIVITYENERGY-EXPENDITUREPROTEIN-KINASESIN-VIVOGENECLK1
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