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Beacon interacts with cdc2/cdc28-like kinases

journal contribution
posted on 2003-04-25, 00:00 authored by Lakshmi Kantham, L Kerr-Bayles, Nathan Godde, Melissa Quick, R Webb, T Sunderland, J Bond, Ken WalderKen Walder, G Augert, Gregory Collier
Previously we found elevated beacon gene expression in the hypothalamus of obese Psammomys obesus. Beacon administration into the lateral ventricle of P. obesus stimulated food intake and body weight gain. In the current study we used yeast two-hybrid technology to screen for proteins in the human brain that interact with beacon. CLK4, an isoform of cdc2/cdc28-like kinase family of proteins, was identified as a strong interacting partner for beacon. Using active recombinant proteins and a surface plasmon resonance based detection technique, we demonstrated that the three members of this subfamily of kinases (CLK1, 2, and 4) all interact with beacon. Based on the known sequence and functional properties of beacon and CLKs, we speculate that beacon could either modulate the function of key regulatory molecules such as PTP1B or control the expression patterns of specific genes involved in the central regulation of energy metabolism.

History

Journal

Biochemical and biophysical research communications

Volume

304

Issue

1

Pagination

125 - 129

Publisher

Elsevier Science

Location

New York, N.Y.

ISSN

0006-291X

eISSN

1090-2104

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2003, Elsevier Science (USA)