File(s) under permanent embargo
Capillary electrophoresis with on-chip four-electrode capacitively coupled conductivity detection for application in bioanalysis
journal contribution
posted on 2001-08-01, 00:00 authored by Rosanne GuijtRosanne Guijt, E Baltussen, G van der Steen, H Frank, H Billiet, T Schalkhammer, F Laugere, M Vellekoop, A Berthold, L Sarro, G W van DedemMicrochip capillary electrophoresis (CE) with integrated four-electrode capacitively coupled conductivity detection is presented. Conductivity detection is a universal detection technique that is relatively independent on the detection pathlength and, especially important for chip-based analysis, is compatible with miniaturization and on-chip integration. The glass microchip structure consists of a 6 cm etched channel (20 microm x 70 microm cross section) with silicon nitride covered walls. In the channel, a 30 nm thick silicon carbide layer covers the electrodes to enable capacitive coupling with the liquid inside the channel as well as to prevent interference of the applied separation field. The detector response was found to be linear over the concentration range from 20 microM up to 2 mM. Detection limits were at the low microM level. Separation of two short peptides with a pI of respectively 5.38 and 4.87 at the 1 mM level demonstrates the applicability for biochemical analysis. At a relatively low separation field strength (50 V/cm) plate numbers in the order of 3500 were achieved. Results obtained with the microdevice compared well with those obtained in a bench scale CE instrument using UV detection under similar conditions.
History
Journal
ElectrophoresisVolume
22Issue
12Pagination
2537 - 2541Publisher
Wiley VCHLocation
Weinheim, GermanyISSN
0173-0835Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
[2001, Wiley VCH]Usage metrics
Categories
No categories selectedKeywords
CalibrationCationsElectric ConductivityElectrodesElectrophoresis, CapillaryEquipment DesignMicrochemistryPeptidesPotassium ChlorideSpectrophotometry, Ultravioletcapillary electrophoresison-chip conductivity detectionScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistryELECTROCHEMICAL DETECTIONSEPARATIONSFABRICATION
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC