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Cloning and characterization of AWP1, a novel protein that associates with serine/threonine kinase PRK1 in vivo
journal contribution
posted on 2000-10-03, 00:00 authored by Wei DuanWei Duan, B Sun, T W Li, B J Tan, M K Lee, T S TeoWe describe the cloning and expression of cDNAs encoding a novel human protein of 208 amino acid residues with a predicted molecular mass of 22.6kDa and its mouse homologue. We name this protein as AWP1 (associated with PRK1). AWP1 is a ubiquitously expressed protein, and the Awp1 gene is switched on during early human and mouse development. When expressed in COS-1 cells, the Myc-tagged AWP1 has an apparent molecular mass higher than that deduced from its amino acid sequence. AWP1 possesses a conserved zf-A20 zinc finger domain at its N-terminal and a zf-AN1 zinc finger domain at its C-terminal. Co-immunoprecipitation experiments revealed that mouse AWP1 specifically interacts with a rat serine/threonine protein kinase PRK1 in vivo. Hence, AWP1 may play a regulatory role in mammalian signal transduction pathways.
History
Journal
GeneVolume
256Issue
1-2Pagination
113 - 121Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0378-1119Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2000, Elsevier Science B.V.Usage metrics
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Keywords
Adaptor Proteins, Signal TransducingAmino Acid SequenceAnimalsBase SequenceBlotting, NorthernCOS CellsCarrier ProteinsChromosome MappingChromosomes, Human, Pair 15Cloning, MolecularDNA, ComplementaryEscherichia coliFemaleGene ExpressionGlutathione TransferaseHumansMiceMolecular Sequence DataPlant ProteinsProtein BindingProtein-Serine-Threonine KinasesProteinsRNA, MessengerRatsReceptor Protein-Tyrosine KinasesRecombinant Fusion ProteinsSequence AlignmentSequence Analysis, DNASequence Homology, Amino AcidTissue DistributionZinc FingersScience & TechnologyLife Sciences & BiomedicineGenetics & HeredityPKNprotein-protein interactionsignal transductionZIPPER-LIKE SEQUENCESRAT-LIVERCELL-DEATHN PKNDOMAINPHOSPHORYLATIONIDENTIFICATIONACTIVATIONEXPRESSIONPhysiologyGenetics
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