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Cloning, expression and immunological characterization of Ory s 1, the major allergen of rice pollen

journal contribution
posted on 1995-10-27, 00:00 authored by H Xu, P Theerakulpisut, N Goulding, Cenk SuphiogluCenk Suphioglu, M B Singh, P L Bhalla
We have isolated and characterized a cDNA clone, Ory s 1, encoding a group-1 allergen of rice pollen. The Ory s 1 protein shows significant sequence identity to the major allergen of rye-grass pollen, Lol p 1. RNA gel blot analysis shows that the Ory s 1 gene is expressed in mature anthers, but not in vegetative or other floral tissues tested. Southern blot analysis indicates that this clone represents a member of a small gene family in rice. Western blot analyses of total rice pollen proteins with the group-1 allergen-specific monoclonal 3A2 and IgE antibodies from grass pollen-allergic patients, revealed the presence of cross-reactive antigenic and allergenic epitopes in Ory s 1.

History

Journal

Gene

Volume

164

Issue

2

Pagination

255 - 259

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

0378-1119

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

1995, Elsevier B.V.