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Combination of Balsamin and Flavonoids Induce Apoptotic Effects in Liver and Breast Cancer Cells

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journal contribution
posted on 2020-10-28, 00:00 authored by P K Ajji, Ken WalderKen Walder, M Puri
Flavonoids such as naringenin, quercetin, and naringin are known to exhibit anticancer properties. In this study, we examined the effects of these flavonoids on cell viability and apoptotic pathways of cancer cells, either singly or in combination with the type 1 ribosome inactivating protein, Balsamin. Treatment with flavonoids (naringenin, quercetin, and naringin) plus Balsamin for 48 h reduced HepG2 and MCF-7 cell viability, increased the activation of caspase-3 and -8, and induced apoptosis through up-regulation of pro-apoptotic genes and down-regulation of anti-apoptotic genes. Out of the three flavonoids tested, the Balsamin-Naringenin and Balsamin-Quercetin combinations appeared to be most effective compared to the Balsamin-Naringin combination. Balsamin combined with flavonoids also activated endoplasmic reticulum (ER)-stress–mediated apoptosis in breast cancer (MCF-7) cells, which was not activated by Balsamin treatment alone. These experimental results showed that Balsamin combined with flavonoids can reduce HepG2 and MCF-7 cells viability and induce apoptosis, which could be considered as a promising therapeutic approach to sensitize cells to Balsamin treatment, thereby improving its efficacy in breast or liver cancer therapy.

History

Journal

Frontiers in Pharmacology

Volume

11

Article number

574496

Pagination

1 - 13

Publisher

Frontiers Media

Location

Lausanne, Switzerland

ISSN

1663-9812

eISSN

1663-9812

Language

eng

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2020, Ajji, Walder and Puri