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Comparative N-Glycoproteomic analysis provides novel insights into the deterioration mechanisms in chicken egg vitelline membrane during high-temperature storage
journal contribution
posted on 2021-02-24, 00:00 authored by Yu Zhou, Ning Qiu, Yoshinori Mine, Russell KeastRussell Keast, Yaqi MengThe weakening of chicken egg vitelline membrane (CEVM) is one of the most important factors influencing egg quality during high-temperature storage. Therefore, a comparative N-glycoproteomic analysis of CEVM after 10 days of storage at 30 °C was performed to explore the roles of protein N-glycosylation in membrane deterioration. In total, 399 N-glycosites corresponding to 198 proteins were identified, of which 46 N-glycosites from 30 proteins were significantly altered. Gene ontology analysis revealed that these differentially N-glycosylated proteins (DGPs) were involved in antibacterial activity, glycosaminoglycan binding, lipid binding, and aminopeptidase activity. Removal of the N-glycans in Mucin-5B may result in a loss of CEVM’s mechanical properties. The N-glycosites enriched in the apolipoprotein B β2 domain in CEVM were significantly changed, which may contribute to lipid composition modifications during storage. Moreover, N-glycosites in several metalloproteases were located within the functional domain or active site region, indicating that the decreased N-glycosylation levels may affect their structural stability, specific substrate binding, or enzyme activity. These findings provide novel insights into the roles of protein N-glycosylation during membrane weakening.
History
Journal
Journal of agricultural and food chemistryVolume
69Issue
7Pagination
2354 - 2363Publisher
American Chemical SocietyLocation
Washington, D.C.Publisher DOI
ISSN
0021-8561eISSN
1520-5118Language
engPublication classification
C1 Refereed article in a scholarly journalUsage metrics
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No categories selectedKeywords
N-glycoproteomechicken egg vitelline membranehigh-temperature storagelabel-freeScience & TechnologyLife Sciences & BiomedicinePhysical SciencesAgriculture, MultidisciplinaryChemistry, AppliedFood Science & TechnologyAgricultureChemistryPROTEOMIC ANALYSISCONVERTING-ENZYMEWHITE PROTEINSYOLK PROTEINSHENS EGGGLYCOSYLATIONIDENTIFICATIONGLYCOPEPTIDESTRENGTHOVOMUCIN
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