wang-correlationbetweenoptical-2005.pdf (544.06 kB)
Correlation between optical and SEM measurements of wool cortical cell size
journal contribution
posted on 2005-01-01, 00:00 authored by Huimin Wang, Xungai WangWool fibres consist of micro to nano scale protein constituents that could be used for innovative applications. While techniques for extracting these constituents or making wool fibres into organic powders have been developed, effectively dispersing the particles and accurately determining their size has been difficult in practice. In this study, an ultrasonic method was employed to disperse cortical cells extracted from wool fibres into an
immersion oil or ethanol. Specimens of the cortical cells were then observed under optical microscopy and scanning electron microscopy, respectively. Cell length and maximum cell diameter were measured to quantify the cell size. The results suggest significant discrepancies exist in the cortical cell size obtained from the two different measurement techniques. The maximum diameter of wool cortical cells obtained from the optical microscope was much larger than that from the scanning electron microscope, while the length was much shorter. A correction factor is given so that cortical cell size obtained from the two measurement techniques can be compared.
immersion oil or ethanol. Specimens of the cortical cells were then observed under optical microscopy and scanning electron microscopy, respectively. Cell length and maximum cell diameter were measured to quantify the cell size. The results suggest significant discrepancies exist in the cortical cell size obtained from the two different measurement techniques. The maximum diameter of wool cortical cells obtained from the optical microscope was much larger than that from the scanning electron microscope, while the length was much shorter. A correction factor is given so that cortical cell size obtained from the two measurement techniques can be compared.
History
Journal
Research journal of textile and apparelVolume
9Issue
3Pagination
23 - 28Publisher
Hong Kong Institution of Textile and ApparelLocation
Hung Hom, Hong KongISSN
1560-6074Language
engPublication classification
C1.1 Refereed article in a scholarly journal; C Journal articleCopyright notice
2005, Research journal of textile and apparelUsage metrics
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