flynn-criticaleffectsof-2018.pdf (860.83 kB)
Critical effects of polar fluorescent probes on the interaction of DHA with POPC supported lipid bilayers
journal contribution
posted on 2018-05-01, 00:00 authored by Kiera R Flynn, Alessandra SuttiAlessandra Sutti, Lisandra L Martin, M Leigh Ackland, Angel TorrieroAngel TorrieroThe understanding of lipid bilayer structure and function has been advanced by the application of molecular fluorophores. However, the effects of these probe molecules on the physicochemical properties of membranes being studied are poorly understood. A quartz crystal microbalance with dissipation monitoring instrument was used in this work to investigate the impact of two commonly used fluorescent probes, 1‑palmitoyl‑2‑{12‑[(7‑nitro‑2‑1,3‑benzoxadiazol‑4‑yl)amino]dodecanoyl}‑sn‑glycero‑3‑phosphocholine (NBD-PC) and 1,2‑dipalmitoyl‑sn‑glycero‑3‑phosphoethanolamine‑n‑(lissamine rhodamine‑B‑sulfonyl) (Lis-Rhod PE), on the formation and physicochemical properties of a 1‑palmitoyl‑2‑oleoyl‑sn‑glycero‑3‑phosphocholine supported lipid bilayer (POPC-SLB). The interaction of the POPC-SLB and fluorophore-modified POPC-SLB with docosahexaenoic acid, DHA, was evaluated. The incorporation of DHA into the POPC-SLB was observed to significantly decrease in the presence of the Lis-Rhod PE probe compared with the POPC-SLB. In addition, it was observed that the small concentration of DHA incorporated into the POPC:NBD-PC SLB can produce rearrangement processes followed by the lost not only of DHA but also of POPC or NBD-PC molecules or both during the washing step. This work has significant implications for the interpretation of data employing fluorescent reporter molecules within SLBs.
History
Journal
Biochimica et biophysica acta (BBA) - biomembranesVolume
1860Issue
5Pagination
1135 - 1142Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
Link to full text
ISSN
0005-2736eISSN
1879-2642Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2018, ElsevierUsage metrics
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Docosahexaenoic acidFluorescent probesQuartz crystal microbalanceSupported lipid bilayer4-Chloro-7-nitrobenzofurazanDocosahexaenoic AcidsFluorescent DyesLipid BilayersMolecular ConformationPhosphatidylcholinesQuartz Crystal Microbalance TechniquesRhodaminesScience & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiophysicsQUARTZ-CRYSTAL MICROBALANCEPOLYUNSATURATED FATTY-ACIDSMOLECULAR-DYNAMICS SIMULATIONSSPIN-LABELED PHOSPHOLIPIDSMEMBRANE PENETRATIONCONTROLLED-TRIALDOUBLE-BLINDQCM-DSUPPLEMENTATION
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