File(s) under permanent embargo
Cytokine and toll-like receptor mRNAs in the nasal-associated lymphoid tissues of cattle during foot-and-mouth disease virus infection
journal contribution
posted on 2006-01-01, 00:00 authored by Z Zhang, J B Bashiruddin, C Doel, J Horsington, S Durand, Soren AlexandersenSoren AlexandersenThe pharyngeal region is known to play an important role in foot-and-mouth disease virus (FMDV) infection in relation to acute disease and viral persistence. In this study, the local mucosal immune response in nasal-associated lymphoid tissue (NALT) of cattle infected with FMDV (strain O UKG 34/2001) was examined. Quantitative "real-time" RT-PCR assays were used to measure mRNA expression of cytokines (IFN-alpha, beta and gamma, IL-2, IL-1alpha and TNF-alpha) and Toll-like receptors (TLR)-3 and -4. NALTs from dorsal soft palate were collected from cattle at 7 days post-infection (dpi) and from carriers and non-carriers at 64 dpi. Expression of IFN-alpha mRNA was significantly greater in NALT during acute disease than in uninfected animals. Increased expression of IFN-gamma and IL-1alpha mRNA was also observed but was much lower than IFN-alpha expression. There was a slight increase in mRNA expression of TNF-alpha and IL-2. During persistence, TNF-alpha mRNA expression in carrier cattle was much higher than in non-carrier cattle. Expression of TLR-4 in NALT during the acute stage of infection was greater than in uninfected animals. Carrier and non-carrier cattle did not differ in respect of expression of TLR-3 and -4 mRNA in NALT.
History
Journal
Journal of comparative pathologyVolume
134Issue
1Pagination
56 - 62Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0021-9975Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2005, Elsevier Ltd.Usage metrics
Categories
No categories selectedKeywords
AnimalsCattleCattle DiseasesFoot-and-Mouth DiseaseLymphoid TissueNoseRNA, MessengerReceptors, CytokineReceptors, InterleukinReceptors, Tumor Necrosis FactorReverse Transcriptase Polymerase Chain ReactionToll-Like Receptor 3Toll-Like Receptor 4Toll-Like ReceptorsScience & TechnologyLife Sciences & BiomedicinePathologyVeterinary Sciencescytokinereal-time RT-PCRviral infectionESOPHAGEAL-PHARYNGEAL FLUIDBUFFALO SYNCERUS-CAFFERALPHA\/BETA INTERFERONGENE-EXPRESSIONCELLSPERSISTENCETRANSMISSIONREPLICATIONVIRULENTSTRAINS
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC