File(s) under permanent embargo
Delivery of abscisic acid to plants using glutathione responsive mesoporous silica nanoparticles
journal contribution
posted on 2018-03-01, 00:00 authored by Dequan Sun, H I Hussain, Zhifeng YiZhifeng Yi, Jim RookesJim Rookes, Lingxue KongLingxue Kong, David CahillDavid CahillAn intracellular glutathione (GSH) responsive phytochemical delivery system based on thiol gated mesoporous silica nanoparticles (MSNs) was developed and tested on the model plant Arabidopsis thaliana. In the present study, monodispersed MSNs with particle diameters of ~20 nm and pore sizes of ~2.87 nm were synthesized and modified. Abscisic acid (ABA), a key phytohormone, was entrapped in the mesopores of MSNs and then the pore entrances of MSNs were covered with decanethiol gatekeepers through GSH-cleavable disulfide linkages. An in vitro release test of ABA from decanethiol gated MSNs proved that there was efficient loading and entrapment of phytochemicals in the absence of a GSH redox trigger. Most importantly, in planta experiments demonstrated that GSH-mediated release of ABA from the pores of MSNs significantly reduced the leaf stomatal aperture and inhibited water loss of treated plants. Moreover, compared with the usage of free ABA, the controlled release of the encapsulated phytohormone from MSNs markedly prolonged the expression of the ABA inducible marker gene (AtGALK2) and finally, improved the drought resistance ability of Arabidopsis seedlings under drought stress. Therefore, the concept of using short-chain molecules as gatekeepers to encapsulate biomolecules in MSNs was demonstrated. The application of MSNs with redox-responsive gatekeepers has been shown in this study to be a potential and efficient technique to deliver phytochemicals into plants and release them in a controllable fashion.
History
Journal
Journal of nanoscience and nanotechnologyVolume
18Issue
3Pagination
1615 - 1625Publisher
American Scientific PublishersLocation
Valencia, Calif.Publisher DOI
ISSN
1533-4880Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2017, American Scientific PublishersUsage metrics
Categories
No categories selectedKeywords
Agrochemical DeliveryControlled ReleaseGlutathione ResponsiveMesoporous Silica NanoparticlesPlant SystemScience & TechnologyPhysical SciencesTechnologyChemistry, MultidisciplinaryNanoscience & NanotechnologyMaterials Science, MultidisciplinaryPhysics, AppliedPhysics, Condensed MatterChemistryScience & Technology - Other TopicsMaterials SciencePhysicsARABIDOPSISSTRESSGATEKEEPERSGROWTHGENEABA
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC