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Development of cell-specific aptamers: recent advances and insight into the selection procedures

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journal contribution
posted on 2017-11-27, 00:00 authored by K Rahimizadeh, H AlShamaileh, M Fratini, M Chakravarthy, M Stephen, Sarah ShigdarSarah Shigdar, R N Veedu
Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice. This approach has also been used for developing aptamers specific to whole cells named Cell-SELEX. Aptamers selected by Cell-SELEX have the potential to act as cell specific therapeutics, cell specific markers or cell specific drug delivery and imaging agents. However, aptamer development is a laborious and time-consuming process which is often challenging due to the requirement of frequent optimization of various steps involved in Cell-SELEX procedures. This review provides an insight into various procedures for selection, aptamer enrichment, regeneration and aptamer-binding analysis, in addition to a very recent update on all aptamers selected by Cell-SELEX procedures.

History

Journal

Molecules

Volume

22

Issue

12

Publisher

M D P I

Location

Basel, Switzerland

eISSN

1420-3049

Language

eng

Publication classification

C Journal article; C1 Refereed article in a scholarly journal

Copyright notice

2017, The Authors