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Development of drug-loaded chitosan-vanillin nanoparticles and its cytotoxicity against HT-29 cells
journal contribution
posted on 2016-01-01, 00:00 authored by P-W Li, Guang Wang, Z-M Yang, Wei DuanWei Duan, Zheng Peng, Lingxue KongLingxue Kong, Q-H WangChitosan as a natural polysaccharide derived from chitin of arthropods like shrimp and crab, attracts much interest due to its inherent properties, especially for application in biomedical materials. Presently, biodegradable and biocompatible chitosan nanoparticles are attractive for drug delivery. However, some physicochemical characteristics of chitosan nanoparticles still need to be further improved in practice. In this work, chitosan nanoparticles were produced by crosslinking chitosan with 3-methoxy-4-hydroxybenzaldehyde (vanillin) through a Schiff reaction. Chitosan nanoparticles were 200-250 nm in diameter with smooth surface and were negatively charged with a zeta potential of - 17.4 mV in neutral solution. Efficient drug loading and drug encapsulation were achieved using 5-fluorouracil as a model of hydrophilic drug. Drug release from the nanoparticles was constant and controllable. The in vitro cytotoxicity against HT-29 cells and cellular uptake of the chitosan nanoparticles were evaluated by methyl thiazolyl tetrazolium method, confocal laser scanning microscope and flow cytometer, respectively. The results indicate that the chitosan nanoparticles crosslinked with vanillin are a promising vehicle for the delivery of anticancer drugs.
History
Journal
Drug deliveryVolume
23Issue
1Pagination
30 - 35Publisher
Taylor & FrancisLocation
Abingdon, Eng.Publisher DOI
ISSN
1071-7544eISSN
1521-0464Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2014, Informa UKUsage metrics
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