File(s) under permanent embargo
Development of reverse transcription-PCR (oligonucleotide probing) enzyme-linked immunosorbent assays for diagnosis and preliminary typing of foot-and-mouth disease: a new system using simple and aqueous-phase hybridization
journal contribution
posted on 2000-12-01, 00:00 authored by Soren AlexandersenSoren Alexandersen, M A Forsyth, S M Reid, G J BelshamA reverse transcription-PCR (RT-PCR)-enzyme-linked immunosorbent assay system that detects a relatively conserved region within the RNA genome of all seven serotypes of foot-and-mouth disease virus (FMDV) has been developed. The high specificity of the assay is achieved by including a rapid hybridization step with a biotin-labeled internal oligonucleotide. The assay is highly sensitive, fast, and easy to perform. A similar assay, based on a highly variable region of the FMDV genome and employing a single asymmetric RT-PCR and multiple hybridization oligonucleotides, was developed to demonstrate the method's ability to type FMDV. Based on our theoretical and practical knowledge of the methodology, we predict that similar assays are applicable to diagnosis and strain differentiation in any system amenable to PCR amplification.
History
Journal
Journal of clinical microbiologyVolume
38Issue
12Pagination
4604 - 4613Publisher
American Society for MicrobiologyLocation
Washington, D.C.ISSN
0095-1137Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2000, American Society for MicrobiologyUsage metrics
Categories
No categories selectedKeywords
AnimalsAphthovirusCapsidCapsid ProteinsCattleEnzyme-Linked Immunosorbent AssayNucleic Acid HybridizationOligonucleotide ProbesReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityScience & TechnologyLife Sciences & BiomedicineMicrobiologyPOLYMERASE-CHAIN-REACTIONSWINE VESICULAR DISEASERT-PCRVIRUSVP1DIFFERENTIATIONEPIDEMIOLOGYTAIWANESESEQUENCESPIGS
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC