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Dielectrophoretic-activated cell sorter based on curved microelectrodes
journal contribution
posted on 2010-08-01, 00:00 authored by Khashayar Khoshmanesh, C Zhang, F Tovar-Lopez, Saeid Nahavandi, Sara Baratchi, A Mitchell, K Kalantar-ZadehThis article presents the numerical and experimental analysis of a dielectrophoretic-activated cell sorter (DACS), which is equipped with curved microelectrodes. Curved microelectrodes offer unique advantages, since they create strong dielectrophoretic (DEP) forces over the tips and maintain it over a large portion of their structure, as predicted by simulations. The performance of the system is assessed using yeast (Saccharomyces cerevisiae) cells as model organisms. The separation of the live and dead cells is demonstrated at different medium conductivities of 0.001 and 0.14 S/m, and the sorting performance was assessed using a second array of microelectrodes patterned downstream the microchannel. Further, microscopic cell counting analysis reveals that a single pass through the system yields a separating efficiency of ~80% at low medium conductivities and ~85% at high medium conductivities.
History
Journal
Microfluidics and nanofluidicsVolume
9Issue
2-3Pagination
411 - 426Publisher
SpringerLocation
Heidelberg, GermanyPublisher DOI
ISSN
1613-4982eISSN
1613-4990Language
engNotes
Published online: 29 December 2009Publication classification
C1 Refereed article in a scholarly journalCopyright notice
2009, Springer-VerlagUsage metrics
Keywords
dielectrophoresismicrofluidicsyeastsortingScience & TechnologyTechnologyPhysical SciencesNanoscience & NanotechnologyInstruments & InstrumentationPhysics, Fluids & PlasmasScience & Technology - Other TopicsPhysicsELECTRIC-FIELDYEAST-CELLSMICROFLUIDIC DEVICESINGLE-CELLRARE CELLSSEPARATIONMANIPULATIONPARTICLESCHIPFLOWMechanical Engineering
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