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Diet during pregnancy is implicated in the regulation of hypothalamic RNA methylation and risk of obesity in offspring
journal contribution
posted on 2018-06-01, 00:00 authored by Antony Kaspi, Ishant Khurana, Mark ZiemannMark Ziemann, Timothy ConnorTimothy Connor, Briana RandallBriana Randall, Paul Zimmet, Ken WalderKen Walder, Assam El-OstaSCOPE: Early life nutrition has long-lasting influence in adults through key mediators that modulate epigenetic states, although the determinants involved that underlie this response remain controversial. Because of the similarities between metabolic, physiological, and endocrine changes and those occurring in human type 2 diabetes, we studied the interaction of diet during pregnancy regulating RNA adenosine methylation (N6-methyladenosine [m6A]) and the transcriptome in Psammomys obesus. METHODS AND RESULTS: Breeding pairs were randomly allocated standard diet (total digestible energy 18 MJ kg-1 ) or low-fat diet (15 MJ kg-1 ). Offspring were weaned onto the low-fat diet at 4 weeks of age and given ad libitum access, resulting in two experimental groups: 1) male offspring of animals fed a low-fat diet and weaned onto the low-fat diet and 2) male offspring of animals fed a standard diet and weaned onto the low-fat diet. Hypothalamic RNA was used to assess m6A by immunoprecipitation. Parental low-fat diet alters the metabolic phenotype in offspring. An association between parental diet and hypothalamic m6A was observed in regulating the expression of FTO and METTL3 in the offspring. CONCLUSIONS: We propose the regulatory capacity is now broadened for the first time to include m6A in developmental programming and obesity phenotype.
History
Journal
Molecular nutrition and food researchVolume
62Issue
14Article number
e1800134Pagination
1 - 10Publisher
Jhn Wiley & SonsLocation
Weinheim, GermanyPublisher DOI
eISSN
1613-4133Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2018, WILEY-VCH Verlag GmbH & Co. KGaA, WeinheimUsage metrics
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