guijt-directcouplingof-2015.pdf (5.3 MB)
Direct coupling of a free-flow isotachophoresis (FFITP) device with electrospray ionization mass spectrometry (ESI-MS)
journal contribution
posted on 2015-09-07, 00:00 authored by J K Park, C D M Campos, P Neužil, L Abelmann, Rosanne GuijtRosanne Guijt, A ManzWe present the online coupling of a free-flow isotachophoresis (FFITP) device to an electrospray ionization mass spectrometer (ESI-MS) for continuous analysis without extensive sample preparation. Free-flow-electrophoresis techniques are used for continuous electrophoretic separations using an electric field applied perpendicular to the buffer and sample flow, with FFITP using a discontinuous electrolyte system to concurrently focus a target analyte and remove interferences. The online coupling of FFITP to ESI-MS decouples the separation and detection timeframe because the electrophoretic separation takes place perpendicular to the flow direction, which can be beneficial for monitoring (bio)chemical changes and/or extensive MS(n) studies. We demonstrated the coupling of FFITP with ESI-MS for simultaneous concentration of target analytes and sample clean-up. Furthermore, we show hydrodynamic control of the fluidic fraction injected into the MS, allowing for fluidically controlled scanning of the ITP window. Future applications of this approach are expected in monitoring biochemical changes and proteomics.
History
Journal
Lab on a chipVolume
15Issue
17Pagination
3495 - 3502Publisher
Royal Society of ChemistryLocation
Cambridge, Eng.Publisher DOI
Link to full text
ISSN
1473-0197eISSN
1473-0189Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2015, The Royal Society of ChemistryUsage metrics
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Citric AcidEquipment DesignFluoresceinIsotachophoresisLab-On-A-Chip DevicesModels, ChemicalSpectrometry, Mass, Electrospray IonizationScience & TechnologyLife Sciences & BiomedicinePhysical SciencesTechnologyBiochemical Research MethodsChemistry, MultidisciplinaryChemistry, AnalyticalNanoscience & NanotechnologyInstruments & InstrumentationBiochemistry & Molecular BiologyChemistryScience & Technology - Other TopicsCAPILLARY-ZONE-ELECTROPHORESISSAMPLE STACKINGCHIPMICROCHIPSYSTEMSSTATEPH
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