dumee-dualfunctional-2018.pdf (2.24 MB)
Dual functional ultrafiltration membranes with enzymatic digestion and thermo-responsivity for protein self-cleaning
journal contribution
posted on 2018-09-19, 00:00 authored by Anbharasi Vanangamudi, Ludovic Dumee, Mikel C Duke, Xing YangControlling surface⁻protein interaction during wastewater treatment is the key motivation for developing functionally modified membranes. A new biocatalytic thermo-responsive poly vinylidene fluoride (PVDF)/nylon-6,6/poly(N-isopropylacrylamide)(PNIPAAm) ultrafiltration membrane was fabricated to achieve dual functionality of protein-digestion and thermo-responsive self-cleaning. The PVDF/nylon-6,6/PNIPAAm composite membranes were constructed by integrating a hydrophobic PVDF cast layer and hydrophilic nylon-6,6/PNIPAAm nanofiber layer on to which trypsin was covalently immobilized. The enzyme immobilization density on the membrane surface decreased with increasing PNIPAAm concentration, due to the decreased number of amine functional sites. An ultrafiltration study was performed using the synthetic model solution containing BSA/NaCl/CaCl2, where the PNIPAAm containing biocatalytic membranes demonstrated a combined effect of enzymatic and thermo-switchable self-cleaning. The membrane without PNIPAAm revealed superior fouling resistance and self-cleaning with an RPD of 22%, compared to membranes with 2 and 4 wt % PNIPAAm with 26% and 33% RPD, respectively, after an intermediate temperature cleaning at 50 °C, indicating that higher enzyme density offers more efficient self-cleaning than the combined effect of enzyme and PNIPAAm at low concentration. The conformational volume phase transition of PNIPAAm did not affect the stability of immobilized trypsin on membrane surfaces. Such novel surface engineering design offer a promising route to mitigate surface⁻protein contamination in wastewater applications.
History
Journal
MembranesVolume
8Issue
3Pagination
1 - 15Publisher
MDPILocation
Basel, SwitzerlandPublisher DOI
Link to full text
ISSN
2077-0375Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2018, the authorsUsage metrics
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thermo-responsiveultrafiltrationenzymesself-cleaningnanofibersScience & TechnologyLife Sciences & BiomedicinePhysical SciencesTechnologyBiochemistry & Molecular BiologyChemistry, PhysicalEngineering, ChemicalMaterials Science, MultidisciplinaryPolymer ScienceChemistryEngineeringMaterials ScienceELECTROSPUN NANOFIBROUS MEMBRANESSURFACE MODIFICATIONIMMOBILIZATIONTRYPSINFABRICATIONCOPOLYMERSSTRATEGIESPARTICLESREMOVAL
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