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Electrokinetic size and mobility traps for on-site therapeutic drug monitoring
journal contribution
posted on 2015-06-15, 00:00 authored by Aliaa I Shallan, Rosanne GuijtRosanne Guijt, Michael C BreadmoreThe extraction of target analytes from biological samples is a bottleneck in analysis. A microfluidic device featuring an electrokinetic size and mobility trap was formed by two nanojunctions of different pore size to extract and concentrate analytical targets from complex samples. The trap was seamlessly coupled with electrophoretic separation for quantitative analysis. The device was applied to the analysis of ampicillin levels in blood within 5 min and a linear response over the range of 2.5-20 μg mL(-1). This covers the recommended levels for treating sepsis, a critical condition with 30 to 50% mortality and unpredicted drug levels. The device provides a new opportunity for on-site therapeutic drug monitoring, which should enable quick and accurate dosing and may save lives in such critical conditions.
History
Journal
Angewandte chemie international editionVolume
54Issue
25Pagination
7359 - 7362Publisher
WileyLocation
Berlin, GermanyPublisher DOI
ISSN
1433-7851eISSN
1521-3773Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2015, Wiley-VCH Verlag GmbHUsage metrics
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No categories selectedKeywords
antibioticsdielectric breakdownelectrokinetic trappingelectrophoresismicrofluidicsAmpicillinAnti-Bacterial AgentsDrug MonitoringEquipment DesignHumansMicrofluidic Analytical TechniquesScience & TechnologyPhysical SciencesChemistry, MultidisciplinaryChemistryBETA-LACTAM ANTIBIOTICSCRITICALLY-ILL PATIENTSSENSORSPATIENTBINDINGCANCER
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