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Evaluation of potential cationic probes for the detection of proline and betaine
journal contribution
posted on 2014-12-01, 00:00 authored by Umme Kalsoom, Michael C Breadmore, Rosanne GuijtRosanne Guijt, Mary C BoyceOsmoregulants are the substances that help plants to tolerate environmental extremes such as salinity and drought. Proline and betaine are two of the most commonly studied osmoregulants. An indirect UV CE method has been developed for simultaneous determination of these osmoregulants. A variety of reported probes and compounds were examined as potential probes for the indirect detection of proline and betaine. Mobility and UV-absorption properties highlighted sulfanilamide as a potential probe for indirect analysis of proline and betaine. Using 5 mM sulfanilamide at pH 2.2 with UV detection at 254 nm, proline and betaine were separated in less than 15 min. The LODs for proline and betaine were 11.6 and 28.3 μM, respectively. The developed method was successfully applied to quantification of these two osmoregulants in spinach and beetroot samples.
History
Journal
ElectrophoresisVolume
35Issue
23Pagination
3379 - 3386Publisher
WileyLocation
Weinheim, GermanyPublisher DOI
eISSN
1522-2683Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2014, WILEY-VCH Verlag GmbHUsage metrics
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No categories selectedKeywords
BetaineCationic probesIndirect detectionOsmoregulantsProlineBeta vulgarisCationsElectrophoresis, CapillaryLimit of DetectionLinear ModelsPlant ExtractsSpinacia oleraceaSulfanilamidesScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistryPERFORMANCE LIQUID-CHROMATOGRAPHYCAPILLARY-ZONE-ELECTROPHORESISINDIRECT UV DETECTIONAMINO-ACID-ANALYSISGLYCINE-BETAINEPLANT-EXTRACTSMETAL IONSDERIVATIZATIONACCUMULATIONSEPARATION
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