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Evaluation of the portable Cepheid SmartCycler real-time PCR machine for the rapid diagnosis of foot-and-mouth disease

journal contribution
posted on 2002-05-01, 00:00 authored by A Hearps, Z Zhang, Soren AlexandersenSoren Alexandersen
The ability of the portable Cepheid SmartCycler real-time PCR machine to detect foot-and-mouth disease (FMD) virus sensitively and accurately was evaluated by comparing the results of the analyses of nasal swab and serum samples from experimentally infected animals with those obtained from the real-time PCR assay currently in use in the laboratory. The results indicated that the ability of the machine to detect viral RNA is greatly affected by the PCR reagents used for the assay. When it was used with PCR beads it was unable to detect weakly positive samples, but when TaqMan core reagents were used for the assay, its sensitivity was significantly increased. The machine could be used for the laboratory-based detection of FMD; however, as with all assays, significant optimisation of assay conditions as well as solid validation of the technique is required.

History

Journal

Veterinary record

Volume

150

Issue

20

Pagination

625 - 628

Publisher

BMJ Publishing Group

Location

London, Eng.

ISSN

0042-4900

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2002, British Veterinary Association