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Experimental foot-and-mouth disease virus infection in white tailed deer
journal contribution
posted on 2012-08-01, 00:00 authored by M Moniwa, C Embury-Hyatt, Z Zhang, K Hole, A Clavijo, J Copps, Soren AlexandersenSoren AlexandersenWhite tailed deer (Odocoileus virginianus) were inoculated with foot-and-mouth disease virus (FMDV) O UKG 11/2001 and monitored for the development of clinical signs, histopathological changes and levels of virus replication. All FMDV-infected deer developed clinical signs starting at 2 days post inoculation and characterized by an increase in body temperature, increased salivation and lesions in the mouth and on the feet. Virus spread to various tissues was determined by quantifying the amount of FMDV RNA using quantitative reverse transcriptase polymerase chain reaction. Virus or viral antigen was also detected in tissues using traditional isolation techniques, enzyme linked immunosorbent assay and immunohistochemistry. Deer-to-cattle transmission of the virus was observed in this experimental setting; however, inoculated deer were not found to become carriers of FMDV.
History
Journal
Journal of comparative pathologyVolume
147Issue
2-3Pagination
330 - 342Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0021-9975eISSN
1532-3129Language
engPublication classification
C Journal article; C1.1 Refereed article in a scholarly journalCopyright notice
2012, ElsevierUsage metrics
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No categories selectedKeywords
AnimalsAnimals, WildCattleDeerDisease Models, AnimalDisease Transmission, InfectiousEnzyme-Linked Immunosorbent AssayFemaleFoot-and-Mouth DiseaseFoot-and-Mouth Disease VirusImmunohistochemistryInfectious Disease Transmission, VerticalMaleRNA, ViralReverse Transcriptase Polymerase Chain ReactionVirus ReplicationScience & TechnologyLife Sciences & BiomedicinePathologyVeterinary Sciencesexperimental infectionpathogenesistransmissionwhite tailed deerBRITISH DEERSEROLOGICAL RESPONSECLINICAL DISEASEWILDLIFEPERSISTENCEANTIBODIESZIMBABWE
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