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Expression of HIV-1 nef in yeast: the 27 kDa Nef protein is myristylated and fractionates with the nucleus
journal contribution
posted on 1993-06-01, 00:00 authored by I G Macreadie, Alister WardAlister Ward, P Failla, A A Azad, E Grgacic, D McPheeThe nef gene of human immunodeficiency virus type 1 (HIV-1) has been expressed in the yeast Saccharomyces cerevisiae to produce native Nef proteins. The proteins of M(r) 27 kDa and 25 kDa, produced by translation from the first and second start codons of the nef gene react with human HIV-1 antisera. Under low-level steady-state expression conditions, Nef27 undergoes myristylation and is targeted to the nuclear fraction while Nef25 is not myristylated and not nuclear localized. When produced rapidly and to high levels, Nef27 is initially present in the cytoplasm as a soluble myristylated protein that later fractionates with the nucleus.
History
Journal
YeastVolume
9Issue
6Pagination
565 - 573Publisher
John Wiley & SonsLocation
Hoboken, N.J.Publisher DOI
ISSN
0749-503XLanguage
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1993, John Wiley & SonsUsage metrics
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Keywords
Base SequenceCell FractionationCell NucleusGene ExpressionHIV-1Molecular Sequence DataMyristic AcidMyristic AcidsRecombinant ProteinsSaccharomyces cerevisiaeNefyeast expressionmyristylationCUP1Science & TechnologyLife Sciences & BiomedicineBiochemistry & Molecular BiologyBiotechnology & Applied MicrobiologyMicrobiologyMycologySACCHAROMYCES-CEREVISIAEHUMAN-IMMUNODEFICIENCY-VIRUSLONG TERMINAL REPEATMUTATIONAL ANALYSISGENE-PRODUCTST-CELLSTYPE-1REPLICATIONREPRESSORVECTORSREGIONMicrobiology
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