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Fattigation-platform nanoparticles using apo-transferrin stearic acid as a core for receptor-oriented cancer targeting
journal contribution
posted on 2017-01-01, 00:00 authored by H H Amin, N M Meghani, C Park, V H Nguyen, T T-D Tran, Phuong TranPhuong Tran, B-J LeeA major hurdle in cancer treatment is the precise targeting of drugs to the cancer site. As many cancer cells overexpress the transferrin receptor (TfR), the transferrin (Tf)-TfR interaction is widely exploited to target cancer cells. In this study, novel amphiphilic apo-Tf stearic acid (TfS) conjugates were prepared and characterized by Fourier transform infrared (FTIR) spectroscopy, matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, and trinitrobenzenesulfonic acid (TNBS) assay. The prepared TfS conjugates were readily self-assembled in water to form nanoparticles (NPs), consisting of TfS as a core of NPs, whose sizes and zeta potentials were determined by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and a particle size analyzer. Hydrophilic water-soluble doxorubicin (DOX) was chosen as a model drug. DOX-loaded TfS NPs (NP+DOX), prepared by the adsorption of DOX on the NP surface via the incubation method, were analyzed for their cell targeting and killing efficiencies in TfR-overexpressing A549 and HCT116 cell lines by MTT assay, confocal microscopy, and fluorescence assisted cell sorting (flow cytometry). The data showed that NP+DOX exhibited improved cancer cell targeting and killing properties compared to that reported for free DOX. Further, the cytotoxic efficiency of NP+DOX was comparable to that of PEGylated liposomal product, Doxil(®), while its cellular uptake was higher than that of Doxil(®). Thus, this novel receptor-based TfS NP drug delivery system has great potential to target TfR-overexpressing cancer cells without off-target effects.
History
Journal
Colloids and surfaces B: biointerfacesVolume
159Pagination
571 - 579Publisher
ElsevierLocation
The NetherlandsPublisher DOI
eISSN
1873-4367Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2017, Elsevier B.V.Usage metrics
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No categories selectedKeywords
Apo-transferrin-stearic acid conjugateDoxorubicinFattigation-platformPhysicochemical characterizationReceptor-oriented cancer targetingSelf-assembled nanoparticlesTransferrinScience & TechnologyLife Sciences & BiomedicinePhysical SciencesTechnologyBiophysicsChemistry, PhysicalMaterials Science, BiomaterialsChemistryMaterials ScienceDRUG-DELIVERYSOLID TUMORIN-VITROCHEMOTHERAPYNANOCARRIERSLIPOSOMESTHERAPYTISSUEWAR
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