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First intron and M-bcr breakpoints are restricted to the lymphoid lineage in Philadelphia positive acute lymphoblastic leukemia
journal contribution
posted on 1990-10-01, 00:00 authored by Jeffrey CraigJeffrey Craig, J M Hawkins, T Yamada, K Ganeshaguru, A B Mehta, L M Secker-WalkerKnowledge of the level of commitment of the target cell in hematological malignancies may have important therapeutic and prognostic implications. Cell lineage involvement was investigated in two cases presenting with acute lymphoblastic leukemia diagnosed on clinical and immunological findings and having the Philadelphia translocation t(9;22)(q34;q11). DNA from cells separated into mononuclear (lymphoid) and granulocytic fractions was hybridized with Philadelphia breakpoint-specific probes. This revealed that the breakpoint giving rise to the Philadelphia chromosome in case 1 was within the major breakpoint cluster region and in case 2 was in the first intron of the BCR gene. Rearrangement was found in the lymphoid but not the granulocyte fraction in each case. It is therefore concluded that the target cell for chromosomal change in these cases was a lymphoid committed progenitor cell, irrespective of breakpoint location.
History
Journal
LeukemiaVolume
4Issue
10Pagination
678 - 681Publisher
Nature Publishing GroupLocation
London, Eng.ISSN
0887-6924Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1990, Nature Publishing GroupUsage metrics
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