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Foot-and-mouth disease viral loads in pigs in the early, acute stage of disease

journal contribution
posted on 2010-01-02, 00:00 authored by C Murphy, J B Bashiruddin, M Quan, Z Zhang, Soren AlexandersenSoren Alexandersen
The progress and pathogenesis of foot-and-mouth disease virus (FMDV) was studied in infected pigs by observing the development of clinical signs in two separate experiments. Viral loads were determined by real-time quantitative RT-PCR in the liver, spleen, cervical lymph node, mandibular lymph node, retropharyngeal lymph node, soft palate, pharynx, tonsil, tongue and skin (coronary band area). Tissue samples were collected from both inoculated and contact-infected pigs at several time points during infection, and blood samples were collected to assess viraemia and its relationship to tissue viral load. Virus first appeared in the lymph nodes, followed by viraemia and then clinical signs. The results suggested that FMDV accumulated in lymphoid tissue up to six hours after infection, in the tissues drained by the mandibular lymph node and tonsil and then disseminated throughout the body where epithelial cells were the favoured sites of replication.

History

Journal

Veterinary record

Volume

166

Issue

1

Pagination

10 - 14

Publisher

BMJ Group

Location

London, Eng.

ISSN

0042-4900

eISSN

2042-7670

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2010, British Veterinary Association