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Functional analysis of a Type-I ribosome inactivating protein balsamin from Momordica balsamina with anti-microbial and DNase activity
journal contribution
posted on 2016-09-01, 00:00 authored by Parminder Kaur Ajji, Ken WalderKen Walder, Munish PuriRibosome inactivating proteins (RIPs) have received considerable attention in biomedical research because of their unique activities towards tumor and virus-infected cells. We extracted balsamin, a type-I RIP, from Momordica balsamina. In the present study, a detailed investigation on DNase activity, antioxidant capacity and antibacterial activity was conducted using purified balsamin. DNase-like activity of balsamin towards plasmid DNA was pH, incubation time and temperature dependent. Moreover, the presence of Mg(2+) (10-50 mM) influenced the DNA cleavage activity. Balsamin also demonstrated reducing power and a capacity to scavenge free radicals in a dose dependent manner. Furthermore, the protein exhibited antibacterial activity against Staphylococcus aureus, Salmonella enterica, Staphylococcus epidermidis and Escherichia coli, which suggests potential utility of balsamin as a nutraceutical.
History
Journal
Plant foods for human nutritionVolume
71Issue
3Pagination
265 - 271Publisher
SpringerLocation
New York, N.Y.Publisher DOI
ISSN
0921-9668eISSN
1573-9104Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2016, SpringerUsage metrics
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