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Generation of recombinant influenza A viruses lacking immunodominant CD8+ T cell epitopes
journal contribution
posted on 2004-06-01, 00:00 authored by S Andreansky, John StambasJohn Stambas, A Gutierrez, G Diaz, P Doherty, S Turner, R WebbyThe induction of a cytotoxic T lymphocyte (CTL) response following influenza infection can lead to the formation of immunity capable of recognizing viruses of a different antigenicity. Our ability to exploit such broadly reactive responses in vaccination strategies is hampered by a lack of understanding on the regulation of CTL responses. In this report, we describe the utilization of reverse genetics to produce a range of recombinant viruses lacking immunodominant murine CTL epitopes. Recombinant viruses lacking the epitopes had indistinguishable growth properties in vitro and in vivo compared with the wild-type virus. Analysis of a primary immune response to these viruses showed that mutation of the anchor-binding residue leads to a loss of a response to that epitope, but no compensating increase in responses to other immunodominant epitopes. The utilization of reverse genetics and the murine model of influenza infection hold great promise for elucidating the factors regulating the CTL response.
History
Journal
International Congress SeriesVolume
1263Pagination
141 - 144Publisher
Elsevier BVLocation
The NetherlandsPublisher DOI
ISSN
0531-5131Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
Copyright 2004 Elsevier B.V. All rights reserved.Usage metrics
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