sertori-genomeediting-2016.pdf (714.48 kB)
Genome editing in zebrafish: a practical overview
journal contribution
posted on 2016-01-01, 00:00 authored by Robert Sertori, Monique Trengove, Faiza Basheer, Alister WardAlister Ward, Clifford LiongueClifford LiongueZebrafish is a powerfulmodel for the study of vertebrate development, being amenable to a wide range of genetic and other manipulations to probe themolecular basis of development and its perturbation in disease. Over recent years, genome editing approaches have become increasingly used as an efficient and sophisticated approach to precisely engineer the zebrafish genome, which has further enhanced the utility of this organism. This review provides a practical overview of genome editing and its application in zebrafish research, including alternate strategies for introducing and screening for specific genetic changes.
History
Journal
Briefings in functional genomicsVolume
15Issue
4Pagination
322 - 330Publisher
Oxford University PressLocation
Oxford, Eng.Publisher DOI
Link to full text
ISSN
2041-2649eISSN
2041-2657Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2015, The AuthorsUsage metrics
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Keywords
zebrafishgenome editingzinc finger nucleasesTALENSsCRISPRHDRScience & TechnologyLife Sciences & BiomedicineBiotechnology & Applied MicrobiologyGenetics & HeredityTALENsZINC-FINGER NUCLEASESTARGETED GENE DISRUPTIONDOUBLE-STRAND BREAKSDNA-BINDINGHOMOLOGOUS RECOMBINATIONIMMUNE-SYSTEMTAL EFFECTORSLARGE-SCALEIN-VIVOMUTAGENESISGenetics
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