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Hyphenated sample preparation-electrospray and nano-electrospray ionization mass spectrometry for biofluid analysis
journal contribution
posted on 2021-06-07, 00:00 authored by I E Mikhail, M Tehranirokh, A A Gooley, Rosanne GuijtRosanne Guijt, M C BreadmoreStand-alone electrospray ionization mass spectrometry (ESI-MS) has been advancing through enhancements in throughput, selectivity and sensitivity of mass spectrometers. Unlike traditional MS techniques which usually require extensive offline sample preparation and chromatographic separation, many sample preparation techniques are now directly coupled with stand-alone MS to enable outstanding throughput for bioanalysis. In this review, we summarize the different sample clean-up and/or analyte enrichment strategies that can be directly coupled with ESI-MS and nano-ESI-MS for the analysis of biological fluids. The overview covers the hyphenation of different sample preparation techniques including solid phase extraction (SPE), solid phase micro-extraction (SPME), slug flow micro-extraction/nano-extraction (SFME/SFNE), liquid extraction surface analysis (LESA), extraction electrospray, extraction using digital microfluidics (DMF), and electrokinetic extraction (EkE) with ESI-MS and nano-ESI-MS.
History
Journal
Journal of Chromatography AVolume
1646Article number
462086Pagination
1 - 18Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0021-9673eISSN
1873-3778Language
engPublication classification
C1 Refereed article in a scholarly journalUsage metrics
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No categories selectedKeywords
Sample preparationStand-alone mass spectrometryElectrospray ionization-mass spectrometry (ESI-MS)Nano-ESI-MSBiological fluidsScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistrySOLID-PHASE MICROEXTRACTION
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