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Identification of T-cell epitopes of Loi p 9, a major allergen of ryegrass (Lolium perenne) pollen

journal contribution
posted on 1996-07-01, 00:00 authored by B Blaher, Cenk SuphiogluCenk Suphioglu, R B Knox, M B Singh, J McCluskey, J M Rolland
T-cell recognition of Loi p 9, a major allergen of ryegrass pollen, was investigated by using a T-cell line and T-cell clones generated from the peripheral blood of an atopic donor. The T-cell line reacted with purified Loi p 9, as well as with crude ryegrass pollen extract, but failed to cross-react with Bermuda grass pollen extract. All of six T-cell clones generated from this line proliferated in response to Loi p 9. Epitope mapping was carried out with a panel of 34 overlapping synthetic peptides, which spanned the entire sequence of the Loi p 9 12R isoform. The T-cell line responded to two of the peptides, Loi p 9 (105-116) and Loi p 9 (193-204), whereas reactivity with one or other of these peptides was shown by five T-cell clones. These two peptides contained sequences consistent with motifs previously reported for major histocompatibility complex class II-restricted peptides. HLA antibody blocking studies showed that presentation of peptide Loi p 9 (105-116) to one T-cell clone was HLA-DR-restricted; this clone expressed a T helper cell phenotype (CD3+, CD4+) and the T-cell receptor αβ. The identification of immunodominant T-cell epitope(s) on allergens is essential for devising safer and more effective immunotherapy strategies, which can interrupt the chain of events leading to allergic disease.

History

Journal

Journal of allergy and clinical immunology

Volume

98

Issue

1

Pagination

124 - 132

Publisher

Elsevier

Location

Amsterdam, The Netherlands

ISSN

0091-6749

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

1996, Mosby, Inc

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