smith-imatinibattentuates-2005.pdf (1.01 MB)
Imatinib attenuates diabetic nephropathy in apolipoprotein E-knockout mice
journal contribution
posted on 2005-02-01, 00:00 authored by M Lassila, K Jandeleit-Dahm, K K Seah, Craig SmithCraig Smith, A C Calkin, T J Allen, M E CooperIn the diabetic kidney, clinical as well as experimental observations have shown an upregulation of growth factors such as PDGF. These studies, however, were not designed to address whether upregulation of PDGF is merely a manifestation of diabetic renal injury or whether PDGF plays an active role in the pathophysiology of diabetic nephropathy. The objectives of this study were first to assess whether PDGF-dependent pathways are involved in the development of diabetic nephropathy and second to determine the effects of PDGF receptor antagonism on this disorder and associated molecular and cellular processes. This study used the diabetic apolipoprotein E-knockout (apoE-KO) mouse, a recently described model of accelerated diabetic nephropathy. Diabetes was induced by injection of streptozotocin in 6-wk-old apoE-KO mice. Diabetic animals received treatment with a tyrosine kinase inhibitor that inhibits PDGF action, imatinib (STI-571, 10 mg/kg per d orally) or no treatment for 20 wk. Nondiabetic apoE-KO mice served as controls. This model of accelerated renal disease with albuminuria as well as glomerular and tubulointerstitial injury was associated with increased renal expression of PDGF-B, proliferating cells, and α-smooth muscle actin-positive cells. Furthermore, there was increased accumulation of type I and type IV collagen as well as macrophage infiltration. Imatinib treatment ameliorated both renal functional and structural parameters of diabetes as well as overexpression of a number of growth factors, collagens, proliferating cells, α-smooth muscle actin-positive cells, and macrophage infiltration within the kidney. Tyrosine kinase inhibition with imatinib seems to retard the development of experimental diabetic nephropathy.
History
Journal
Journal of the American society of nephrologyVolume
16Issue
2Pagination
363 - 373Publisher
American Society of NephrologyLocation
[Washington, D.C.]Publisher DOI
Link to full text
ISSN
1046-6673eISSN
1533-3450Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2005, American Society of NephrologyUsage metrics
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