desilva-immunologicalcompar-2018.pdf (1010.83 kB)
Immunological comparison of native and recombinant hen's egg yolk allergen, chicken serum albumin (Gal d 5), produced in Kluveromyces lactis
journal contribution
posted on 2018-06-12, 00:00 authored by Chamika De Silva, Pathum Dhanapala, Samuel King, Timothy Doran, Mimi Tang, Cenk SuphiogluCenk SuphiogluChicken serum albumin (CSA) is a hen's egg yolk allergen causing IgE-mediated allergy. The objective of this study was to produce a recombinant version of CSA and compare its IgE reactivity to natural CSA (nCSA). CSA was cloned and expressed as a soluble fraction in the yeast Kluyveromyces lactis (K. lactis) protein expression system. The gene encoding CSA was amplified with a C-terminal hemagglutinin epitope tag by polymerase chain reaction (PCR) and cloned into the pKLAC2 expression vector prior to transforming into K. lactis. Recombinant CSA (rCSA) was purified by immunoprecipitation. Twenty-one patients allergic to hen's egg white were examined for sensitisation against nCSA. 38% of patients were found to be sensitised to CSA based on Western immunoassay. Immunoglobulin E (IgE) binding capacity of rCSA and nCSA was analysed by ELISA using sera from patients sensitised to CSA. Levels of IgE-binding were similar for both the recombinant and the natural CSA, indicating the existence of similar epitopes. rCSA produced in this study is a potential candidate to be used in component-resolved diagnosis (CRD) of egg yolk allergy. The usefulness of rCSA in CRD of egg yolk allergy warrants further characterisation using sera from patients with allergy to hen's egg yolk in future studies.
History
Journal
NutrientsVolume
10Issue
6Article number
757Pagination
1 - 11Publisher
MDPILocation
Basel, SwitzerlandPublisher DOI
Link to full text
eISSN
2072-6643Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2018, the authorsUsage metrics
Categories
No categories selectedKeywords
allergyegg yolk allergensrecombinant allergenschicken serum albuminAllergensAnimalsAntibody SpecificityBiomarkersChickensEgg HypersensitivityEgg Proteins, DietaryEpitopesHumansImmunoglobulin EKluyveromycesRecombinant ProteinsSerum AlbuminScience & TechnologyLife Sciences & BiomedicineNutrition & Dietetics
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC