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In-plane alloy electrodes for capacitively coupled contactless conductivity detection in poly(methylmethacrylate) electrophoretic chips
journal contribution
posted on 2013-11-01, 00:00 authored by Adam J Gaudry, Michael C Breadmore, Rosanne GuijtRosanne GuijtA simple method for producing PMMA electrophoresis microchips with in-plane electrodes for capacitively coupled contactless conductivity detection is presented. One PMMA plate (channel plate) is embossed with the microfluidic and electrode channels and lamination bonded to a blank PMMA cover plate of equal dimensions. To incorporate the electrodes, the bonded chip is heated to 80 °C, above the melting point of the alloy (≈ 70 °C) and below the glass transition temperature of the PMMA (≈ 105 °C), and the molten alloy drawn into the electrode channels with a syringe before being allowed to cool and harden. A 0.5 mm diameter stainless steel pin is then inserted into the alloy filled reservoirs of the electrode channels to provide external connection to the capacitively coupled contactless conductivity detection detector electronics. This advance provides for a quick and simple manufacturing process and negates the need for integrating electrodes using costly and time-consuming thin film deposition methods. No additional detector cell mounting structures were required and connection to the external signal processing electronics was achieved by simply slipping commercially available shielded adaptors over the pins. With a non-optimised electrode arrangement consisting of a 1 mm detector gap and 100 μm insulating distance, rapid separations of ammonium, sodium and lithium (<22 s) yielded LODs of approximately 1.5-3.5 ppm.
History
Journal
ElectrophoresisVolume
34Issue
20-21Pagination
2980 - 2987Publisher
WileyLocation
Weinheim, GermanyPublisher DOI
eISSN
1522-2683Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2013, WILEY-VCH VerlagUsage metrics
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No categories selectedKeywords
C4DElectrode integrationElectrophoresisMicrochipPMMAAlloysAmmonium CompoundsElectric ConductivityElectrodesElectrophoresis, MicrochipEquipment DesignLimit of DetectionLithiumPolymethyl MethacrylateSodiumScience & TechnologyLife Sciences & BiomedicinePhysical SciencesBiochemical Research MethodsChemistry, AnalyticalBiochemistry & Molecular BiologyChemistry(CD)-D-4ON-A-CHIPCAPILLARY-ELECTROPHORESISGLASS CHIPSMICROFLUIDIC CHIPSSAMPLE INJECTIONINORGANIC-IONSFABRICATIONDEVICESSYSTEMSSEPARATIONS
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