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Interfacing cell-based assays in environmental scanning electron microscopy using dielectrophoresis
journal contribution
posted on 2011-01-01, 00:00 authored by Khashayar Khoshmanesh, J Akagi, Saeid Nahavandi, K Kalantar-Zadeh, Sara Baratchi, D Williams, J Cooper, D WlodkowicDevelopment of the dielectrophoretic (DEP) live cell trapping technology and its interfacing with the environmental scanning electron microscopy (ESEM) is described. DEP microelectrode arrays were fabricated on glass substrate using photolithography and lift-off. Chip-based arrays were applied for ESEM analysis of DEP-trapped human leukemic cells. This work provides proof-of-concept interfacing of the DEP cell retention and trapping technology with ESEM to provide a high-resolution analysis of individual nonadherent cells.
History
Journal
Analytical chemistryVolume
83Issue
8Pagination
3217 - 3221Publisher
American Chemical SocietyLocation
Washington, D. C.ISSN
0003-2700eISSN
1520-6882Language
engPublication classification
C1 Refereed article in a scholarly journalCopyright notice
2011, American Chemical Society.Usage metrics
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