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Intracellular drug delivery by sulfatide-mediated liposomes to gliomas
journal contribution
posted on 2006-10-10, 00:00 authored by K Shao, Q Hou, Wei DuanWei Duan, M Go, K Wong, Q T LiWe described here a liposomal carrier system in which the targeting ligand was sulfatide, a glycosphingolipid known to bind several extracellular matrix (ECM) glycoproteins whose expression was highly up-regulated in many tumors. In vitro experiments with human glioma cell lines demonstrated that robust intracellular uptake of the liposomes depended specifically on the presence of sulfatide as the key liposomal component. Significant amount of the liposomes remained largely intact in the cytoplasm for hours following their internalization. When anticancer drug doxorubicin (DOX) was encapsulated in such liposomes, most of the drug was preferably delivered into the cell nuclei to exert its cytotoxicity. Use of this drug delivery system to deliver DOX for treatment of tumor-bearing nude mice displayed much improved therapeutic effects over the free drug or the drug carried by polyethylene glycol (PEG)-grafted liposomes. Our results demonstrate a close link between effective intracellular uptake of the drug delivery system and its therapeutic outcome. Moreover, the sulfatide-containing liposomes (SCL) may represent an interesting ligand-targeted drug carrier for a wide spectrum of cancers in which sulfatide-binding ECM glycoproteins are expressed.
History
Journal
Journal of controlled releaseVolume
115Issue
2Pagination
150 - 157Publisher
Elsevier B.V.Location
Amsterdam, The NetherlandsPublisher DOI
ISSN
0168-3659Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
2006, Elsevier B.V.Usage metrics
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