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Investigation of the pathogenesis of transplacental transmission of Aleutian mink disease parvovirus in experimentally infected mink
journal contribution
posted on 1996-03-01, 00:00 authored by S Broll, Soren AlexandersenSoren AlexandersenThe transplacental transmission of Aleutian mink disease parvovirus (ADV) was studied in experimental infection of 1-year-old female non-Aleutian mink. The ADV-seronegative female mink were inoculated with ADV prior to mating or after the expected implantation of the embryos during pregnancy. A group of uninfected females served as a control group. Animals from each group were killed prior to or shortly after parturition. The in situ hybridization technique with radiolabeled strand-specific RNA probes was used to determine target cells of virus infection and virus replication. In both infected groups, ADV crossed the endotheliochorial placental barrier, although animals infected before mating already had high antibody titers against ADV at the time of implantation. The percentage of dead and resorbed fetuses was much higher in dams infected before mating. In the placentae of these mink, virus DNA and viral mRNA were detected in cells in the mesenchymal stroma of the placental labyrinth and hematoma but only occasionally in the cytotrophoblast of the placental hematoma. Placentae of animals infected during pregnancy showed in addition very high levels of virus and also viral replication in a large number of cytotrophoblast cells in the placental hematoma, which exhibited distinct inclusion bodies. In both groups, neither virus nor virus replication could be detected in maternal endothelial cells or fetal syncytiotrophoblast of the placental labyrinth. Fetuses were positive for virus and viral replication at high levels in a wide range of tissues. Possible routes of transplacental transmission of ADV and the role of trophoblast cells as targets for viral replication are discussed.
History
Journal
Journal of virologyVolume
70Issue
3Pagination
1455 - 1466Publisher
American Society for MicrobiologyLocation
Washington, D.C.ISSN
0022-538XeISSN
1098-5514Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1996, American Society for MicrobiologyUsage metrics
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No categories selectedKeywords
Acute DiseaseAleutian Mink DiseaseAleutian Mink Disease VirusAnimalsFemaleInfectious Disease Transmission, VerticalMaleMinkPlacentaPregnancyPregnancy Complications, InfectiousVirus LatencyScience & TechnologyLife Sciences & BiomedicineVirologyADULT MINKINSITU HYBRIDIZATIONPORCINE PARVOVIRUSVIRUSMACROPHAGESREPLICATIONREPRODUCTIONCELLSDNA
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