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Isolating conifer DNA: a superior polysaccharide elimination method

journal contribution
posted on 2003-03-01, 00:00 authored by Tamsyn CrowleyTamsyn Crowley, Morley Muralitharan, T Stevenson
Genomic DNA was isolated from frozen needles of maturePinus radiata clones using a modified extraction technique incorporating cetyltrimethylammonium bromide (CTAB) for cell lysis. A high sodium chloride concentration (2 M) was used at 2 stages of the extraction procedure to eradicate polysaccharides, yielding pure genomic DNA suitable for restriction enzyme digestion and PCR amplification. Extractions were scaled down to suit 1.5-mL Eppendorf tubes, allowing easier handling and enhanced sterility.

History

Journal

Plant molecular biology reporter

Volume

21

Issue

1

Pagination

1 - 4

Publisher

Springer Netherlands

Location

Amsterdam, The Netherlands

ISSN

0735-9640

eISSN

1572-9818

Language

eng

Notes

SpringerLink Date Saturday, December 15, 2007

Publication classification

C1 Refereed article in a scholarly journal

Copyright notice

2003, International Society for Plant Molecular Biology.

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