Deakin University
Browse
kong-isolatingmotile-2022.pdf (4.7 MB)

Isolating motile sperm cell sorting using biocompatible electrospun membranes

Download (4.7 MB)
journal contribution
posted on 2022-01-01, 00:00 authored by R D Katigbak, Ludovic DumeeLudovic Dumee, Lingxue KongLingxue Kong
AbstractMotility is an indicator of sperm cell viability due to higher probability in swimming through the female reproductive tract and undergo fertilization with the egg cell. Centrifugation method is a technique to process high volume semen and isolate motile sperm cells but decreases the biochemical integrity of spermatozoa due to the contact with reactive oxygen species (ROS) from dead cells released during centrifugation. This study uses solution electrospun poly(ε-caprolactone) membranes as an alternative in isolating motile spermatozoa by utilizing a rationally designed 3D printed module set up, providing the same benefits as commercially available techniques with minimal processing time, and bypassing the centrifugation step to provide higher quality sperm cells. The membranes, with nominal pore size distributions ranging from 5 to 6 µm are highly porous structures suitable for establishing baseline data for sperm cell sorting by motility. The proposed method allows for isolation of motile sperm cells with 74% purity, while decreasing the processing time by 98% when compared to centrifugation techniques. This novel approach provides a facile method for isolating motile spermatozoa directly from frozen semen samples without any pretreatments and is easily scalable for small and medium scale farms as well as larger industries.

History

Journal

Scientific Reports

Volume

12

Article number

ARTN 6057

Pagination

1 - 8

Publisher

Springer Nature

Location

Berlin, Germany

ISSN

2045-2322

eISSN

2045-2322

Language

English

Publication classification

C1 Refereed article in a scholarly journal

Usage metrics

    Research Publications

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC