wade-isolationanddetectionof-2016.pdf (1.4 MB)
Isolation and detection of microRNA from the egg of chickens
journal contribution
posted on 2016-05-23, 00:00 authored by B Wade, Michelle Cummins, A Keyburn, Tamsyn CrowleyTamsyn CrowleyBACKGROUND: The egg is a vital part of the chicken developmental process and an important protein source for humans. Despite the chicken egg being a subject of intense research little attention has been given to the role of microRNAs within the egg. FINDINGS: We report a method for the reproducible and reliable isolation of miRNA from the albumen and yolk of chicken eggs. We also report the detection via real-time PCR of a number of miRNAs from both of these biological fluids. CONCLUSIONS: These findings provide an interesting look into the chicken egg and raise questions as to the role that miRNAs maybe playing in the chicken egg. This method of detecting miRNAs in chicken eggs will allow researchers to investigate the presence of an additional level of epigenetic programming in chick development previously unknown and also how this impacts the nutritional value of eggs for human consumption.
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Journal
BMC research notesVolume
9Article number
283Pagination
1 - 6Publisher
BioMed CentralLocation
London, Eng.Publisher DOI
eISSN
1756-0500Language
engPublication classification
C Journal article; C1 Refereed article in a scholarly journalCopyright notice
2016, The AuthorsUsage metrics
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